sterile α motif (sam) domains (Stryker)
90
Structured Review
Stryker
sterile α motif (sam) domains
Sterile α Motif (Sam) Domains, supplied by Stryker, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+%CE%B1+motif+(sam)+domains/sterile+%CE%B1+motif++sam++domains/pmc02776498-41-6-23
Average 90 stars, based on 1 article reviews
Sterile α Motif (Sam) Domains, supplied by Stryker, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+%CE%B1+motif+(sam)+domains/sterile+%CE%B1+motif++sam++domains/pmc02776498-41-6-23
Average 90 stars, based on 1 article reviews
sterile α motif (sam) domains - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules"
Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules
Journal: Journal of Cell Science
doi: 10.1242/jcs.047266
Figure Legend Snippet: KazrinE gene structure and interaction with LAR. (A) Intron-exon organisation of the human kazrin gene. Arrows, translation initiation sites; TGA, translation termination codon; AATAAA, polyadenylation signal. Dashed arrow shows generation of kazrinE by alternative splicing. (B) Liprin phylogenetic tree. Dm, Drosophila melanogaster; Gg, Gallus gallus; Hs, Homo sapiens; Sc, Saccharomyces cerevisiae; Xl, Xenopus laevis. Sc_STE11 is included as a SAM-domain-containing outlier. (C) RT-PCR using primers to detect the kazrin isoforms shown or GAPDH as an input-level control. HK, human keratinocytes; –/–, no RNA. (D) Schematic diagram of kazrinA and kazrinE. KazrinE is shown with the kazrinA N-terminus (A). lz, leucine-zipper-like domain; nls, putative nuclear-localisation sequence; SAM, sterile α motif. The location of peptide immunogens is shown. (E) 293T cells transiently co-transfected with HA-kazrinE (KE) and GFP-LAR (LAR) were lysed in the presence (+PI) or absence (–PI) of phosphatase inhibitors and either examined directly by anti-HA immunoblotting (lysate) or following immunoprecipitation with anti-GFP (GFP IP). As a control, anti-GFP alone was added to beads (–). (F) A431 cells were transiently transfected with GFP (left-hand lane) or HA-kazrinE, serum starved for 16 hours and either left untreated (–) or treated with EGF (+) for 1 hour prior to lysis in the presence of phosphatase inhibitors. Upper panels: HA-kazrinE was immunoprecipitated and immunoblotted with anti-phosphotyrosine (p-Tyr) or anti-HA. Arrow indicates phosphorylated kazrinE. Lower panels: western blots of total-cell lysates probed with antibodies to HA or α-tubulin (α-Tub) as controls for transfection and loading, respectively. (G) Keratinocytes retrovirally transduced with HA-kazrinE were transiently transfected with GFP or GFP-LAR (green), immunolabelled with anti-HA antibody (red) and counterstained with DAPI (blue). The boxed region is shown at higher magnification in the right-hand panel. Scale bar: 10 μm.
Techniques Used: Alternative Splicing, Reverse Transcription Polymerase Chain Reaction, Control, Sequencing, Sterility, Transfection, Western Blot, Immunoprecipitation, Lysis, Transduction
Related Articles
Sterility:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of Alternative Splicing:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of Reverse Transcription Polymerase Chain Reaction:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of Control:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of Sequencing:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of Transfection:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of Western Blot:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of Immunoprecipitation:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of Lysis:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of Transduction:Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of |